Introduction
Understanding and optimizing enzymatic reactions is a critical need across various industries, including clinical diagnostics, drug development, and biotechnology. These reactions are foundational to many processes, and precise control over enzymatic activity can lead to more efficient and cost-effective outcomes. Customers in these sectors require reliable methods to study enzyme kinetics, particularly when it comes to analyzing how variables like substrate concentration and temperature influence reaction rates.
PerkinElmer’s fluorometric assay addresses this need by providing detailed insights into enzyme kinetics, using the Michaelis-Menten model. The study, focusing on horseradish peroxidase (HRP), revealed that reaction rates increase with substrate concentration until enzyme saturation. The FL 6500 fluorescence spectrometer, coupled with a Peltier controller, proved effective in delivering these critical insights for process optimization.
Like what you're reading?
To view the full content, please answer a few questions.